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By Jesse P. Greenstein, John T. Edsall
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Additional info for Amino Acids, Proteins and Cancer Biochemistry
Sequence of oxidized bovine ribonuclease and location of the disulfide bridges in the native molecule. There are many indications in the literature that the native molecule is folded in a compact form and is held together not only by covalent bonds but also by hydrogen bonds. For example, Car lisle and Scouloudi (1951) obtained dimensions of 18 X 30 X 48 Â. from X-ray studies of wet ribonuclease crystals. 09 X 106 for the hydrodynamic parameter ß (Scheraga and Mandelkern, 1953), which suggests that the axial ratio may not be very different from unity.
Coombs, T. , and Neurath, H. (1958). J. Am. Chem. Soc. 80, 4750. Vallée, B. , Rupley, J. , Coombs, T. , and Neurath, H. (1960). / . Biol. Chem. 235, 64. Williams, R. J. P. (1959). In "The Enzymes" (P. D. Boyer, H. Lardy, and K. , Vol. I, p. 391. Academic Press, New York. Yang, J. , and Doty, P. (1957). J. Am. Chem. Soc. 79, 761. Chromatographie Evaluation of Protein Mixtures HERBERT A. SOBER and ELBERT A. S. Department of Health, Education and Welfare, Bethesda, Maryland W E FEEL IT TO BE particularly appropriate that the Chromatographie evaluation of protein mixtures be discussed at this Jesse P.
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