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By Hidesaburo Hanafusa (Eds.)

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SFFV-specific neutralizing antibodies can be prepared and tested for inhibition of SFFV-induced proliferation. This has not been examined to date since available neutralizing sera also neutralize the ecotropic helper virus needed for infection. Finally, it should be determined whether cells present in the spleen at early stages of SFFV-induced polyclonal proliferation are necessarily infected, or whether uninfected spleen cells can be stimulated by factors produced by neighboring infected cells.

Binding was enhanced by the presence of calcium ions, and was not sensitive to neuraminidase or alkylating and reducing agents. Receptor activity could not be readily extracted from cells by chaotropic agents, but was removed by detergents. Moreover, the receptor was sensitive to both proteases and phospholipase C. These results suggest that the receptor is probably an intrinsic membrane protein and requires lipid for either its activity or integrity in the cellular membrane. A number of conflicting reports concerning the molecular characterization of putative ecotropic MuLV receptors have appeared.

Virol. 60, 9 1 0 - 9 1 9 . Rein, A. (1982). Interference grouping of murine leukemia viruses: A distinct receptor for MCF-recombinant viruses in mouse cells. Virology 120, 251-257. Richardson, C. , and Choppin, P. W. (1983). Oligopeptides that specifically inhibit membrane fusion by paramyxoviruses: Studies on the site of action. Virology 131, 518-532. Richardson, C. , and Choppin, P. W. (1980). Specific inhibition of paramyxovirus and myxovirus replication by oligopeptides with amino acid sequences similar to those at the N-termini of the F !

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