Download Cryo-EM, Part C: Analyses, Interpretation, and Case Studies by Grant Jensen PDF
By Grant Jensen
This quantity is devoted to an outline of the tools, samples, protocols, and analyses that belong to cryo-EM. It emphasizes the relatedness of the tips, intrumentation, and strategies underlying all cryo-EM methods which permit practictioners to simply flow among them. inside each one part, the articles are ordered in accordance with the commonest symmetry of the pattern to which their equipment are applied.* Includes time-tested core methods and new innovations applicable to any researcher * tools incorporated are important to either established researchers and rookies to the sector * correct heritage and reference details given for approaches can be utilized as a consultant
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Additional info for Cryo-EM, Part C: Analyses, Interpretation, and Case Studies
SIDEROVSKI VOLUME 391. Liposomes (Part E) Edited by NEJAT DU¨ZGU¨NES¸ VOLUME 392. RNA Interference Edited by ENGELKE ROSSI VOLUME 393. Circadian Rhythms Edited by MICHAEL W. YOUNG VOLUME 394. Nuclear Magnetic Resonance of Biological Macromolecules (Part C) Edited by THOMAS L. JAMES VOLUME 395. Producing the Biochemical Data (Part B) Edited by ELIZABETH A. ZIMMER AND ERIC H. ROALSON VOLUME 396. Nitric Oxide (Part E) Edited by LESTER PACKER AND ENRIQUE CADENAS VOLUME 397. Environmental Microbiology Edited by JARED R.
This step requires a cubic density map with an even number of voxels. Additionally, to maximize compatibility with other modeling programs, the origin of the segmented subunit should be reset to zero. py from EMAN2 can be used with the “clip” and “origin” options. py in EMAN2), (2) as a plug-in to UCSF’s Chimera, or (3) within Gorgon. The first two options require the installation of EMAN or EMAN2, while Gorgon’s version of SSEHunter is built-in. In each of these cases, the user is required ˚ /pixel) and a to provide the resolution in A˚, the sampling of the map (A threshold corresponding to the highest isosurface value at which all density of the segmented subunit appears to be connected (obtained visually, $ 2–4s above the mean density).
The helices required only slight rotations and translations. 9. 8 A˚ Ca– Ca distances. The density skeleton was used to identify potential paths through the density. 10. In the Mm-cpn homology model, $30 amino acids were unresolved at the termini. In Coot, Ca atoms were added consecutively to the model starting at the ends of the model until the N-terminus was reached or until the density was not visible (C-terminus). The final model for the Mm-cpn monomer contained residues 1–532 (Fig. 6C). 11.